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Guide: Mixing Peptides with Bacteriostatic Water

Peptide Reconstitution Guide

Peptide reconstitution is the process of adding bacteriostatic water to a lyophilized peptide powder in order to create a liquid solution that can be measured accurately with an insulin syringe.

Most research peptides are supplied in 5mg, 10mg, or 15mg vials and must be mixed before they can be measured into syringe units.


Basic Reconstitution Formula

The concentration of a peptide solution depends on two factors:

• The amount of peptide in the vial
• The amount of bacteriostatic water added

For example:

10mg peptide + 2mL bacteriostatic water

This creates a solution where:

1mL = 5mg
1 unit = 0.05mg


Common Reconstitution Example

10mg vial mixed with 2mL bacteriostatic water:

Dose Syringe Units
100mcg 2 units
250mcg 5 units
500mcg 10 units
1mg 20 units

How to Reconstitute a Peptide

  1. Clean the vial stopper with an alcohol pad
  2. Draw bacteriostatic water into an insulin syringe
  3. Slowly inject the water into the vial
  4. Let the liquid run down the side of the vial
  5. Gently swirl (do not shake)

This creates a fully dissolved peptide solution.


Use the Peptide Calculator

For exact calculations based on your vial size and water amount, use the interactive tool below:

Peptide Dosage Calculator

This calculator converts peptide doses into syringe units instantly.

Open the Peptide Calculator


Research Disclaimer

This information is provided for educational and research purposes only and does not constitute medical advice.


Peptide Research Tools

Peptide Specific Calculators

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