NAD+ Dosage & Protocol Guide: Injections, IV Therapy, and Reconstitution Explained

NAD+ (Nicotinamide Adenine Dinucleotide)

NAD+ is a critical coenzyme involved in cellular energy production, mitochondrial function, and DNA repair. Levels decline with age, making NAD+ supplementation a key strategy in longevity, metabolic health, and recovery protocols.

How NAD+ Works

Cellular Energy ↑ → Mitochondrial Function ↑ → DNA Repair ↑ → Cellular Resilience ↑

NAD+ plays a central role in ATP production and activates pathways involved in cellular repair and longevity, including sirtuins. Supporting NAD+ levels helps improve energy, recovery, and metabolic efficiency.

NAD+ Protocol (Subcutaneous)

Week 1

Dose50 mg

Units30 units (0.30 mL)

Week 2

Dose75 mg

Units45 units (0.45 mL)

Weeks 3–16

Dose100 mg

Units60 units (0.60 mL)

Frequency: Once daily
Cycle Length: 8–16 weeks

Alternative Clinical Use

• IV infusion: 250–500 mg weekly (clinical setting)
• IM injection: 50 mg up to 3× weekly
• SubQ: 30–100 mg daily

When & Where to Inject

• Best time: Morning (energy support)
• SubQ sites: abdomen, thigh, upper arm
• Rotate injection sites
• Stay hydrated before and after dosing

Reconstitution & Dosing (500 mg Vial)

Vial 500 mg

Water 3.0 mL bacteriostatic

Concentration 166.7 mg/mL

Conversion 1 unit = 1.67 mg

Reconstitution Steps

Draw 3.0 mL bacteriostatic water

Inject slowly down vial wall

Gently swirl (do not shake)

Refrigerate immediately

Storage

Lyophilized: −20°C (−4°F)

Reconstituted: 2–8°C (36–46°F)

Protect from light

Use Window

Best used within 14 days

Avoid freeze–thaw cycles

Benefits

• Increases cellular energy (ATP production)
• Supports mitochondrial function
• Enhances recovery and endurance
• Promotes DNA repair and longevity pathways
• Improves mental clarity and focus
• Supports metabolic health

Potential Side Effects

• Flushing or warmth sensation
• Mild nausea (higher doses)
• Fatigue or overstimulation initially
• Injection site irritation
• Limited long-term data

Stacking Strategies

• Energy Stack: NAD+ + MOTS-c
• Longevity Stack: NAD+ + Epitalon
• Mitochondrial Support: NAD+ + SS-31

Important Disclaimer

NAD+ protocols vary based on individual needs. This content is for educational purposes only and does not constitute medical advice.

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